In§addition to updating important core techniques this third edition presents new§chapters on assessing leukocyte involvement in angiogenesis, lymphatic cell and§pericyte isolation techniques, spheroid and arterial ring based in vitro assays, and on§pericyte involvement in angiogenesis. Angiogenesis, Third Edition also guides§readers through new protocols on chorioallantoic membrane models, corneal§pocket assays to assess angio- and lymphangiogenesis, models of muscle§angiogenesis, and use of zebrafish embryos to study vascular angiogenesis and§senescence. Written in the highly successful Methods in Molecular§Biology series format, chapters include introductions to their§respective topics, lists of the necessary materials and reagents, step-by-step,§readily reproducible laboratory protocols, and tips on troubleshooting and§avoiding known pitfalls.§§Authoritative§and practical, Angiogenesis, Third Edition provides not only a§practical handbook for key techniques, but also an informative and enjoyable§read for all those interested, no matter how directly, in angiogenesis.§
